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. 2023 May 23;11(6):1511. doi: 10.3390/biomedicines11061511

Figure 3.

Figure 3

(A,B) Effects of DAPTA on GM-CSF- and MCP-1-expressing CD40+ B cells in the spleen cells were analyzed using flow cytometry. (C,D) Effects of DAPTA on Gm-csf and Mcp-1 mRNA expression levels were analyzed using RT-PCR in brain tissue. (E) Flow cytometry representative dot plots of CD40+MCP-1+ expression are shown in one mouse from each group. Naive SJL/J mice were treated with saline (naive), EAE mice were treated with saline (EAE), and EAE mice were treated with DAPTA (EAE+DAPTA), 0.01 mg/kg, i.p., once daily from day 14 to day 42. All data are shown as mean ± SD (n = 6). * p < 0.05.