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. 2023 May 27;12(6):777. doi: 10.3390/biology12060777

Figure 6.

Figure 6

Gold nanoparticles (AuNPs) modulated expression of micrRNA-204-5p and MMP-9 in MCF-7 breast cancer cells. (A) AuNPs upregulate the expression of hsa-miR-204-5p in PMA-stimulated MCF-7 breast cancer cells. MCF-7 cells were pretreated with AuNPs (0.2–0.4 μg/mL) for 2 h and stimulated by PMA (0.1 µM) for 24 h. Unstimulated MCF-7 cells were used as an experimental negative control. Comparative expression of hsa-miR-204-5p was determined by TaqMan assays using RNU6B expression as an endogenous control. * p < 0.05 versus unstimulated MCF-7 cells; # p < 0.05 versus *; @ p < 0.01 versus *; @ p < 0.05 versus #. (B) AuNPs inhibit the expression of MMP-9 mRNA in PMA-stimulated MCF-7 breast cancer cells. MCF-7 cells were pretreated with AuNPs (0.2–0.4 μg/mL) for 2 h and stimulated by PMA (0.1 µM) for 24 h. Unstimulated MCF-7 cells were used as an experimental negative control. Comparative expression of MMP-9 mRNA was determined by TaqMan assays using GAPDH expression as an endogenous control. * p < 0.001 versus unstimulated MCF-7 cells; # p < 0.05 versus *; @ p < 0.01 versus *; @ p < 0.05 versus #. (C) AuNPs inhibit MMP-9 protein production in the culture medium of PMA-stimulated MCF-7 breast cancer cells. MCF-7 cells were pretreated with AuNPs (0.2–0.4 μg/mL) for 2 h and stimulated by PMA (0.1 µM) for 24 h, and the levels of MMP-9 in the culture medium were determined by human MMP-9 immunoassays. A culture medium from unstimulated MCF-7 cells was used as an experimental negative control. * p < 0.0001 versus unstimulated MCF-7 cells; # p < 0.05 versus *; @ p < 0.01 versus *; @ p < 0.05 versus #. (D) AuNPs upregulate the expression of hsa-miR-204-5p in MCF-7 cells transected with anti-miR-204-5p. Anti-miR-204-5p transfected MCF-7 cells were incubated with AuNPs (0.2–0.4 μg/mL) for 24 h. MCF-7 cells transfected with anti-miR-control were used as an experimental negative control. Comparative expression of hsa-miR-204-5p was determined by TaqMan assays using RNU6B expression as an endogenous control. #p < 0.01 versus anti-miR-control transfected MCF-7 cells; # p < 0.05 versus @; @ p < 0.01 versus *; * p < 0.01 versus #. (E) AuNPs inhibit the expression of MMP-9 mRNA in MCF-7 cells transected with anti-miR-204-5p. Anti-miR-204-5p transfected MCF-7 cells were incubated with AuNPs (0.2–0.4 μg/mL) for 24 h. MCF-7 cells transfected with anti-miR-control were used as an experimental negative control. Comparative expression of MMP-9 mRNA was determined by TaqMan assays using GAPDH expression as an endogenous control. # p < 0.001 versus anti-miR-control transfected MCF-7 cells; # p < 0.05 versus @. (F) AuNPs inhibit MMP-9 protein production in MCF-7 cells transected with anti-miR-204-5p. Anti-miR-204-5p transfected MCF-7 cells were incubated with AuNPs (0.2–0.4 μg/mL) for 24 h. MCF-7 cells transfected with anti-miR-control were used as an experimental negative control. MMP-9 levels in the culture medium were determined by human MMP-9 immunoassays. # p < 0.001 versus anti-miR-control transfected MCF-7 cells; # p < 0.05 versus @. The data shown are mean ± SD of three independent experiments.