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. 2023 May 27;12(6):777. doi: 10.3390/biology12060777

Figure 9.

Figure 9

AuNPs inhibit PMA-induced transcription factor NF-κB activation via hsa-miR-204-5p in MCF-7 cells transfected with anti-miR-204-5p. (A) AuNPs inhibit PMA-induced NF-κBp65 activation in MCF-7 cells transfected with anti-miR-204-5p determined by NF-κBp65 transcription factor kit (Abcam, Waltham, Boston, USA). The NF-κBp65 positive control was supplied with the kit. Transfection of MCF-7 cells with anti-miR-ctr was used as a negative control, whereas treatment of MCF-7 cells with parthenolide (a marker of NF-κB inhibitor) was used as a positive control of the experiment. The data shown are mean ± SD of three independent experiments. The data differ without a common letters, p < 0.05. (B) Parthenolide upregulates hsa-miR-204-5p expression in anti-miR-204-5p-transfected MCF-7 cells stimulated with PMA. Anti-miR-204-5p transfected MCF-7 cells were pretreated with parthenolide for 2 h and stimulated by PMA (0.1 µM) for 24 h. Unstimulated/non-transfected MCF-7 cells were used as an experimental negative control. Comparative expression of hsa-miR-204-5p was determined by TaqMan assays using RNU6B expression as an endogenous control. # p < 0.01 versus non-transfected MCF-7 cells; # p < 0.05 versus ##. (C) Parthenolide inhibits MMP-9 mRNA expression in anti-miR-204-5p-transfected MCF-7 cells stimulated with PMA. Anti-miR-204-5p transfected MCF-7 cells were pretreated with parthenolide for 2 h and stimulated by PMA (0.1 µM) for 24 h. Unstimulated/non-transfected MCF-7 cells were used as an experimental negative control. Comparative expression of MMP-9 mRNA was determined by TaqMan assays using GAPDH expression as an endogenous control. # p < 0.05 versus non-transfected MCF-7 cells; ## p < 0.05 versus #. (D) Parthenolide inhibits MMP-9 production in the culture medium of anti-miR-204-5p-transfected MCF-7 cells stimulated with PMA. Anti-miR-204-5p transfected MCF-7 cells were pretreated with parthenolide for 2 h and stimulated by PMA (0.1 µM) for 24 h. The culture medium obtained from unstimulated/non-transfected MCF-7 cells was used as an experimental negative control. MMP-9 production levels in the culture medium were determined by MMP-9-specific immunoassays. # p < 0.01 versus non-transfected MCF-7 cells; ## p < 0.05 versus #. The data shown are mean ± SD of three independent experiments.