Figure 8.

ROS-induced fluorescence (arbitrary units), by H₂-DCF-DA assay, measured at different time points (every 5 min) in HaCaT control cells (Ctrl) and cells exposed for 1 h to different amounts of H₂O₂ (0.5, 1, and 2.5 mM) in the absence and in the presence (24 h of pre-incubation) of C. limon leaf essential oil (LLEO) at the dose of 10 μg/mL (0.5 + LLEO, 1 + LLEO, 2.5 + LLEO). Data were presented as mean ± SD (n = 9). At each time point, significant differences (p < 0.001) were observed for all oxidized samples (in the absence and in the presence of LLEO) versus the respective Ctrl. Evaluation of the statistical significance of differences between groups was performed by one-way ANOVA followed by the Bonferroni Multiple Comparisons Test.