Table 1.
Sequences of LAMP primers for detecting PEDV, PoRV and PBoV.
| Target Gene | Primer | Sequence (5′–3′) | Modification | Length |
|---|---|---|---|---|
| PEDV-gp6 NC_003436.1 |
F3 | GGTACTTGCAAACAACGCTG | — | 218 bp |
| B3 | TCTTTGCGCCTTCTTTAGCA | — | ||
| FIP | TCAATTCGCTCACCACGGCGTTTTCAAGGGGAATAAGGACCAGC | — | ||
| BIP | ACTACCTCGGAACAGGACCTCATTTTACCCAGAAAACACCCTCAGT | — | ||
| LF | AGCGAATTTGCTCATTCCAGTA | 5′ Biotin | ||
| LB | GACCTCCGTTATAGGACTCGT | 5′ Digoxin | ||
| PBoV-vp1
NC_023673.1 |
F3 | CAACACCACAGTCGGGTAAC | — | 228 bp |
| B3 | TTTCCCTCCCCCATCTGG | — | ||
| FIP | GCTCTGGACGCCAATTCTTGGTTTTTATTTACGCAACGGGACAAGT | — | ||
| BIP | GCAACAAGATGAGAGCCGACGTTTTTGGCATGGTTTCGTAGTAGCT | — | ||
| LF | TCCCATTCAATTTCGCAGGAG | 5′ Biotin | ||
| LB | TACAAAATCAACGCCGATGGAGGAT | 5′ Cy5 | ||
| PoRV-vp6 KC113249.1 |
F3 | CATGCTACTGTCGGACTT | — | 198 bp |
| B3 | CAAGTTATCTTCTCTTGAAGGT | — | ||
| FIP | GCCGTTACATTTGCCAATAAAGTTTTTTTGAACTGAATCTGCAGTTTGT | — | ||
| BIP | TTCGTCAGGAATATGCTATACCAGTTTTTGAATAATTGGTAACCAGCTCTG | — | ||
| LF | CGTCCGCAAGCACAGATTC | 5′ Biotin | ||
| LB | GACCAGTATTTCCACCAGGTATG | 5′ ROX |