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. 2023 Jun 6;13(12):1892. doi: 10.3390/ani13121892

Table 3.

Sequences of primers used in qPCR and relevant references containing qPCR conditions.

Gene Primer Sequence Reference
β-actin F GGACTTTGAGCAGGAGATGG [23]
β-actin R ATGATGGAGTTGTAGGTGGTCT
igm F ACCTTAACCAGCCGAAAG [24]
igm R TGTCCCATTGCTCCAGTC
cd4 F CCTGCTCATCCACAGCCT [24]
cd4 R CTTCTCCTGGCTGTCTGA
cd8 F AGTCGTGCAAAGTGGGA [24]
cd8 R GGTTGCAATGGCATACAG
il1-β F ACATTGCCAACCTCATCATCG [25]
il1-β R TTGAGCAGGTCCTTGTCCTTG
tnf-α F GGGGACAAACTGTGGACTGA [26]
tnf-α R GAAGTTCTTGCCCTGCTCTG
il8 F CACAGACAGAGAAGGAAGGAAAG [23]
il8 R TGCTCATCTTGGGGTTACAGA
tgf-β F TCTGAATGAGTGGCTGCAAG [27]
tgf-β R GGTTTCCCACAATCACAAGG
tlr9 F GCAACCAGTCCTTCCACATT [28]
tlr9 R AAACCCAGGGTAAGGGTTTG

F—forward, R—reverse.