Figure 3.

Quantitative analysis of the rate of TPP⁺ influxes, Ca²⁺ retention capacity, and mitochondrial swelling in rat heart mitochondria isolated from the rats of every group. (a)—CRC reflects the threshold Ca²⁺ concentration required to open the mPTP; (b)—the rate of TPP⁺ influx reflecting Ca²⁺-induced drop ΔΨm (V^TPP+_in, nmol min⁻¹ mg⁻¹ of protein); (c)—time to reach half-maximal light scattering (T_1/2) reflecting the rate of swelling of mitochondria. The data are presented as the means ± SDs of five independent experiments. * p < 0.05 a significant difference compared with control (group 1); # p < 0.05 a significant difference relative to ISO (group 3).