Figure 3.

Effect of DIFs (100 nM) on chemotaxis in Ax2, gbpB⁻, and regA⁻ cells. (A) Ax2, (B) gbpB⁻, and (C) regA⁻ cells were starved for 6 h in shake-culture, and cell droplets were spotted on PB agar containing 3 mM caffeine (Control) plus 100 nM DIF compounds. Cells were assayed for chemotaxis toward the indicated doses of cAMP; 10 cell droplets were examined for each cAMP concentration. Data are presented as the mean ± SD of triplicate measurements (n = 3) for one experiment. * and † signify statistically significant inhibition and promotion of chemotaxis, respectively; p < 0.05 versus Control.