Figure 3.

Mice were treated with vehicle or antibiotic mixture (abx) and sacrificed 2 weeks (wks) later; otherwise, mice were treated with abx and sacrificed 2, 4, or 6 weeks (wks) post antibiotic wash-out (w/o). Immunofluorescence on whole-mount preparations of the distal ileum for βIII-tubulin (neuronal markers) was performed. Representative images of 4 independent experiments; 10 independent fields per animal were examined. Scale bars: 75 µm (a). Western blot analysis of HuC/D and S-100β expression on protein extracts obtained from the LMMP. β-actin was used as a loading control. Representative images of 3 independent experiments are provided (b). Protein signals of HuC/D and S-100β were determined through densitometric analysis of Western blots reported in (b). Data are reported as mean ± SD. number of mice for each experimental group = 3 mice per group. ^c denotes p < 0.05 vs. vehicle-treated mice. Light grey: vehicle; dark grey: abx; intermediate grey: wash-out (c).