Table 1.

The number of enzymes missed from the reconstructed carbohydrate and glycan metabolic pathways of the A. fulica snail. Additional enzymes were successfully filled into the pathways after three curation steps (manual curation, sequence similarity search, and conserved domain and structural prediction). Numbers represent the number of enzymes, and ✓ indicates that all enzymes are present in that particular pathway.

Pathways	Number of Missing Enzymes	Number of Enzymes That Passed the Filtering Criteria	Number of Enzymes Added After the First Gap Filling	Number of Enzymes Added After the Second Gap Filling	Completed Pathways/Remained Missing Enzymes
Carbohydrate metabolic pathways
Pentose phosphate	12	8	4	-	✓
Pentose and glucuronate interconversions	9	8	-	1	✓
Fructose and mannose metabolism	7	4	3	-	✓
Galactose metabolism	3	2	1	-	✓
Ascorbate metabolism	5	4	1	-	✓
Starch and sucrose metabolism	5	3	2	-	✓
Amino sugar and nucleotide sugar metabolism	3	3	-	-	✓
Inositol phosphate metabolism	8	5	3	-	✓
Glyoxylate and dicarboxylate metabolism	11	8	3	-	✓
Propanoate metabolism	6	3	3	-	✓
Butanoate metabolism	6	3	3	-	✓
Pyruvate metabolism	5	4	-	1	✓
Glycolysis/gluconeogenesis	4	1	3	-	✓
Glycan metabolic pathways
Chondroitin sulfate/dermatan sulfate biosynthesis	4	-	2	-	2
Galactose type of O-glycan biosynthesis	1	-	1	-	✓
Glycosylphosphatidylinositol (GPI) biosynthesis	4	-	1	-	3
Mannose type O-glycan biosynthesis CORE 1	5	4	1	-	✓
Mannose type O-glycan biosynthesis CORE 2	3	1	2	-	✓
Mucin type of O-glycan biosynthesis	2	1	1	-	✓
N-glycan biosynthesis	1	-	1	-	✓
Glycohormone type of N-glycan biosynthesis	3	-	2	-	✓
Hyaluronan biosynthesis	1	-	-	-	1
Keratan sulfate biosynthesis	2	-	-	2	✓
Total number of enzymes	110	48	38	10	6