Figure 4.
Specificity of the CRISPR/Cas12a system for detecting E. coli O157:H7. (A) Real-time fluorescence detection assay using the CRISPR/Cas12a-FQ detector for different bacterial strains. (B) Fluorescence generated from different bacterial strains. Numbers 1–7 represent E. coli O157:H7 (CICC 24187), E. coli (ATCC 25922), E. coli (ATCCBAA2452), S. aureus (CICC 10306), P. aeruginosa (CICC 21636), S. typhimurium (CICC 22956), and L. monocytogenes (CICC 21635), respectively. Significant differences between groups were tested with the independent samples t-test (**** represents p < 0.0001).