Figure 1.

Analysis of pre- and post-operative N-glycans in CRC by RPLC-FD-MS. Serum samples were taken pre- and post-operation from the same patient. Each sample underwent fluorescent labeling and sialic acid derivatization N-glycan workflow followed by RPLC-FD-MS measurement. Three quantification approaches were used for data processing, namely FD, MS, and a combination of these two approaches via FD-MS. Direct N-glycan and derived glycosylation traits were determined in order to perform data analysis and comparison of the two groups. Adapted with permission from Moran, A.B., et al., 2022. Analytical Chemistry, 94(18), pp. 663–6648 [16]. Copyright 2022 American Chemical Society.