Figure 4.

Early activation signaling events were determined by measuring CD3ζ and Lck phosphorylation. (a) Representative microscopic images following 15 min of effector–tumor co-culturing. (b) Quantitative image analysis of CD3ζ and Lck phosphorylation. The area of the immune synapse, the average and the integrated intensity of the phospho-specific label in the synapse are plotted showing the distribution of individual data points as well as their mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.