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. 2023 Jun 7;15(12):3081. doi: 10.3390/cancers15123081

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Kinetic analysis of in vitro killing of the various CAR T cells. Killing of JIMT-1 target cells was determined using the Electric Cell-substrate Impedance Sensing method. The effector/target ratio was 1:1. Impedance was monitored for 25 h. Averaged traces were normalized to impedance measured at the start of treatment, and these normalized impedances were normalized to the corresponding value of the non-transduced (NT) T cell control at every time point. The graph represents mean ± SD from N = 2 independent experiments with 2 technical replicates.