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. 2023 Jun 8;11(6):1666. doi: 10.3390/biomedicines11061666

Figure 4.

Figure 4

Combination of ABT-199 and MIK665 results in increased cleavage and activity of caspase-3 in HCC cell lines. (A–C) Western blot analyses of (cleaved) caspase-3. Shown is a representative western blot. Cells were treated with either one of the two BH3-mimetics ABT-199 or MIK665 alone or a combination of both inhibitors. DMSO was used as vehicle control. (D,E) Activity assays of caspase-3/7 in HCC cell lines (Hep3B (red), HepG2 (blue), and Huh7 (green)) after 4 h of treatment with either ABT-199 or MIK665 alone or in combination at the indicated concentrations (the dotted line separates the single treatment from the combination treatment with ABT-199 and MIK665). Data show a specific increase in activity compared to DMSO-treated controls (n = 3, mean ± SEM). (F–H) Western blot analyses of (cleaved) PARP. Shown is a representative western blot. Cells were incubated with ABT-199 and MIK665 at the indicated concentrations as single or combined treatment. DMSO was used as vehicle control. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 compared to single drug treatment).