Figure 3.

Protective effects of MLT against cell injury in NP cells induced by M1-type polarization of Mφ. NP cells were treated with different CMs collected from the LPS-stimulated RAW 264.7 Mφs in the presence or absence of MLT for 24 h, then followed by different analysis. (A–D) Reduction of oxidative stress by MLT in NP cells. (A) MLT decreased the level of MDA. (B) MLT increased the level of GSH. (C,D) The ROS levels were declined after treatment with different concentrations of MLT. The levels of GSH and MDA in NP cells were determined by ELISA (n = 3). The intracellular ROS levels in NP cells were measured by flow cytometry assay. Data are expressed as mean ± standard deviation. The two groups among the five groups are compared by using an independent t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.