Figure 1.

Overview of the four most important components to consider when establishing a gut-on-a-chip (GOC). The decision workflow is depicted above the four quadrants. (1) The biological question is important for the characteristics the GOC must comply to, determining the choices to make in quadrants 2, 3 and 4. (2) Fabrication and materials used for the chip slide, membrane and extracellular matrix (ECM). (3) Tissue engineering entails the different cell types that are cultured on the GOC. (4) Environmental factors and read-out parameters. Figure created with BioRender.com, accessed on 19 April 2023.