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. 2023 Jun 16;15(12):3211. doi: 10.3390/cancers15123211

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of cluster 3 as potential transition or cancer stem cell cluster. (A) UMAP of cell cluster identities. (B) Violin plots of gene signature of cluster 3 alone and when compared with muscle cell clusters. (C) Monocle trajectory map indicating a zoomed-in UMAP of cluster 5 (LGACC) (indicated as 1 on the graph) as the primary and cluster 3 (indicated as 2 on the graph) cells coming from the malignant cluster. (D) UMAPs of genes of interest for cluster 3 found in trajectory analysis.