Figure 2.

Fluorescence (Tubulin Tracker Green) images showing examples of axonal growth for cortical neurons cultured on PDL-coated PDMS surfaces with periodic micropatterns. (a) Example of growth image for neurons, acquired at t_culture = 10 h after plating. The direction of the micropatterns is shown by the x-axis. The figure also shows the main structural components of a neuronal cell. Cortical neurons typically grow a long process (axon) and several shorter processes (dendrites). The axons are identified by their morphology, and the growth cone is located at the tip of the axon. (b) Example of growth for neurons imaged at t_culture = 50 h after plating. The image shows a high degree of axonal alignment along the direction x of the micropatterns.