Table 1.
Specific activities (μmol/min per mg) of Nobo from An. gambiae with alternative GST substrates. Initial reaction rates at 30 °C were determined spectrophotometrically by published procedures. The values are based on triplicate measurements.
| Substrate | WT | D111N | S9A | H40N | R116A |
|---|---|---|---|---|---|
| CDNB | 24.7 ± 3.4 | 26.2 ± 4.8 | 1.8 ± 0.2 | 14.0 ± 0.5 | 3.3 ± 0.1 |
| DCNB | 16.4 ± 1.9 | 14.7 ± 0.5 | 0.49 ± 0.03 | 5.0 ± 0.1 | 2.34 ± 0.02 |
| CuOOH | 5.0 ± 0.5 | 3.8 ± 0.4 | 0.05 ± 0.01 | 0.9 ± 0.1 | 0.16 ± 0.02 |
| Allyl-ITC | 3.3 ± 0.4 | 3.4 ± 0.5 | 0.2 ± 0.1 | 2.7 ± 0.3 | 2.0 ± 0.2 |
| Phenethyl-ITC | 0.4 ± 0.1 | 1.2 ± 0.3 | 0.04 ± 0.01 | 0.34 ± 0.02 | 0.1 ± 0.04 |
| 5-AD | 245 ± 8 | 0.8 ± 0.1 | 16.0 ± 2.0 | 69.3 ± 3.7 | 48.1 ± 1.2 |
| 5-PD | 49.7 ± 6.1 | 0.6 ± 0.2 | 5.3 ± 0.5 | 23.7 ± 4.1 | 14.7 ± 1.4 |