Table 1.
Quality controls of ASL mRNA and lipid nanoparticle ASL mRNA (ASL mRNA-LNP).
| Name (LNP-ASL mRNA) |
mRNA Length (nt) | Codon Opt. | Bioanalyzer Integrity (%) |
dsRNA per 1 µg mRNA (pg) |
Capping % | Z-Avg (nm) | PDI | % Encap. |
|---|---|---|---|---|---|---|---|---|
| CDS1 | 1862 | Wild-type | 89 | <1 | 84 | 73 | 0.06 | 88 |
| CDS2 | 1862 | GC-rich | 92 | <1 | 80 | 76 | 0.07 | 87 |
| CDS3 | 1862 | Non-GC-rich | 93 | <1 | 87 | 77 | 0.05 | 89 |
| CDS4 | 1885 | GC-rich | 73 | Pass | 99 | 72 | 0.09 | 93 |
CDS, coding sequence; GC-rich, guanine, cytosine-rich; nt, nucleotide; codon opt., codon optimization; dsRNA, double-stranded RNA; Z-avg, Z-average size; PDI, polydispersity index; encap., particle encapsulation.