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. 2023 Jun 18;15(12):3234. doi: 10.3390/cancers15123234

Figure 4.

Figure 4

Mean of the number of spermatozoa in mice injected with DMSO (Ctrl), MTT, MTT and testosterone together (MTT + Test) and testosterone (Test). Results are expressed as number of spermatozoa per epididymis (a). Quantification of sperm with abnormal chromatin structures calculated by the evaluation of the parameter DNA fragmentation index (DFI). A high %DFI correspond to a large percentage of damaged germ cells. Results are expressed as arbitrary units (a.u.) (b). Values are represented as the mean ± s.e.m. of three independent experiments with total animal numbers of Ctrl = 12, MTT = 12, MTT + Test = 7 and Test = 4. Statistical analysis was performed using ANOVA followed by the Tukey–Kramer test. ** p < 0.01, *** p < 0.001 vs. MTT.