. Author manuscript; available in PMC: 2023 Jun 27.

Published in final edited form as: ACS Catal. 2022 Oct 20;12(21):13501–13505. doi: 10.1021/acscatal.2c03383

Table 1.

Yields and enantioselectivities for bromoetherification of 1 using representative FDHs^a


Entry	FDH	Time (h)	Yield (%)^a	e.r.^b
1	4V+S	18	66	79:21
2	6TL T52V	18	48	36:64
3	4PL	18	68	91:9
4	4PL	2	88	91:9

Reactions conducted using 1 mM 1, 5 mM NaBr, 5 mol% FDH, 100 μM NAD and FAD, 20 mM glucose, 1 mM glutathione, 2.5 μM flavin reductase (RebF),³¹ 9 U/mL glucose dehydrogenase (GDH), and 35 U/mL catalase (Cat.) in 200 mM tricine, pH 7.5.

Yields determined by LC/MS relative to internal standard; selectivities determined by chiral HPLC.