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. 2023 Jun 7;12(12):1580. doi: 10.3390/cells12121580

Figure 3.

Figure 3

Recombinant expression and purification of the BFSP1 protein domains used in these studies. (A). BFSP1 and the domains 434–665, 434–548 and 494–550 were cloned into pET vectors, and their expression was induced by the addition of IPTG. In each of the panels, a sample of the uninduced, followed by a sample after a 3 h induction with IPTG and the subsequent affinity purified proteins are shown. The same markers (M) were used, although the acrylamide concentration changed; 10% (w/v) for BFSP1 1–665; 12% (w/v) for BFSP1 434–665 and 434–548; and lastly, 15% (w/v) for BFSP1 494–550. Markers correspond to 250, 130, 100, 70, 55, 35, 25, 15, and 10 kDa, respectively. (B). Bovine BFSP1 purified from bovine lenses.