Table 3.
Change in the Heliquest Discrimination Factor as a result of reported sequence variants in the 434–464 sequence of BFSP1, which includes the amphipathic helix (grey highlight). In this table, three sequence variants (Q436R, Y445C and R446S) reported for human BFSP1 (https://www.uniprot.org/uniprotkb/Q12934/entry#disease_variants, accessed on 10 May 2023) are analysed using the Heliquest algorithm (https://heliquest.ipmc.cnrs.fr, accessed on 10 May 2023). The values for the Hydrophic moment (HM) for z (net charge) and then used to calculate the Discrimination Factor (DF) for the wild type and three variants as indicated. Those sequences where a hydrophobic face is predicted are indicated (grey highlight), and DFs that are either higher or lower than the wild type of sequence are indicated (bold).
| BFSP1 434–454 (Wild Type) | Wild Type | Q436R | Y445C | R446S | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptide Sequence Scan | HM | z | DF | HM | z | DF | HM | z | DF | DM | z | DF |
| GGQISKGFGKLYRKVKEK | 0.53932 | 5 | 2.15911808 | 0.56706 | 6 | 2.51530464 | 0.55949 | 5 | 2.17815856 | 0.49211 | 4 | 1.78455184 |
| GQISKGFGKLYRKVKEKV | 0.59755 | 5 | 2.2140872 | 0.62717 | 6 | 2.57204848 | 0.61913 | 5 | 2.23445872 | 0.55201 | 4 | 1.84109744 |
| RISKGFGKLYRKVKEKVR | 0.63442 | 6 | 2.57889248 | 0.66175 | 7 | 2.934692 | 0.65431 | 6 | 2.59766864 | 0.58691 | 5 | 2.20404304 |
| ISKGFGKLYRKVKEKVRS | 0.62845 | 6 | 2.5732568 | 0.62845 | 6 | 2.5732568 | 0.64803 | 6 | 2.59174032 | 0.58061 | 5 | 2.19809584 |
| SKGFGKLYRKVKEKVRSP | 0.57526 | 6 | 2.52304544 | 0.57526 | 6 | 2.52304544 | 0.59602 | 6 | 2.54264288 | 0.52872 | 5 | 2.14911168 |
| KGFGKLYRKVKEKVRSPK | 0.56039 | 7 | 2.83900816 | 0.56039 | 7 | 2.83900816 | 0.57893 | 7 | 2.85650992 | 0.51154 | 6 | 2.46289376 |
| GFGKLYRKVKEKVRSPKE | 0.54099 | 5 | 2.16069456 | 0.54099 | 5 | 2.16069456 | 0.55961 | 5 | 2.17827184 | 0.49222 | 4 | 1.78465568 |
| FGKLYRKVKEKVRSPKEP | 0.54667 | 5 | 2.16605648 | 0.54667 | 5 | 2.16605648 | 0.56336 | 5 | 2.18181184 | 0.49629 | 4 | 1.78849776 |
| GKLYRKVKEKVRSPKEPE | 0.4226 | 4 | 1.7189344 | 0.4226 | 4 | 1.7189344 | 0.43641 | 4 | 1.73197104 | 0.37039 | 3 | 1.33964816 |
| KLYRKVKEKVRSPKEPET | 0.41416 | 4 | 1.71096704 | 0.41416 | 4 | 1.71096704 | 0.42877 | 4 | 1.72475888 | 0.36327 | 3 | 1.33292688 |
| LYRKVKEKVRSPKEPETP | 0.35341 | 3 | 1.32361904 | 0.35341 | 3 | 1.32361904 | 0.36259 | 3 | 1.33228496 | 0.29968 | 2 | 0.94289792 |
| YRKVKEKVRSPKEPETPT | 0.28225 | 3 | 1.256444 | 0.28225 | 3 | 1.256444 | 0.29517 | 3 | 1.26864048 | 0.22951 | 2 | 0.87665744 |
| RKVKEKVRSPKEPETPTE | 0.26427 | 2 | 0.90947088 | 0.26427 | 2 | 0.90947088 | 0.26427 | 2 | 0.90947088 | 0.21098 | 1 | 0.52916512 |