Figure 7.

Gene expression induced by rhizobia in shoots. (A) Heat map of average fragments per kilobase of transcript per million mapped reads (FPKMs) of genes showing increased expression during the first 72 h of nodulation. Each row is independently scaled from minimum to maximum values; underlying data in in Supplemental Dataset 2, Sheet F6. Eight genes were induced in shoots of wild type plants. Seven of these were also induced in shoots of AON mutant plants sunn-4 and rdn1-2. Three additional genes were induced only in sunn-4. Two genes that were also induced in nodulating roots are indicated by “**”. Graphical representation of selected genes is shown in (B,C) with FPKMs (black dots) and their means (red lines) from three biological replicates. Shading is the standard error of the mean. (B) Transcription factor gene Medtr3g111880 was induced by 24 h in shoots of wild-type but not sunn-4, while in roots expression was induced in all three lines. (C) Three genes induced in shoots of sunn-4.