Figure 8.

Enhanced DTH by challenge injection of Fc−scGITRL. (A) Schematic diagram of the experimental schedule for inducing DTH response, immunized with mBSA and CFA, and challenged with mBSA (210 µg/mouse) only (group 1, G1) or mBSA (210 µg/mouse) plus Fc−scGITRL (10 µg/mouse) (G2). (B) Footpad swelling on day 8. Data are average and standard error of the mean of seven mice per group. (C) Histological analysis of footpads on day 8. Histological sections from PBS or mBSA injected footpad in G1 (left) and PBS or mBSA plus Fc−scGITRL injected footpad in G2 (right) were stained with hematoxylin and eosin (H&E). Scale bar, 100 µm. (D) mBSA-specific T cell proliferative responses. Pooled spleen cells from mice immunized and challenged with mBSA only (G1) or mBSA plus Fc−scGITRL (G2) were cultured with indicated concentrations of mBSA for 3 d. Cell proliferation was assessed by MTT assay. The average and the standard error of the mean of three wells are shown. * p < 0.05 (Student t test).