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. 2023 Jun 7;12(12):1575. doi: 10.3390/cells12121575

Figure 1.

Figure 1

Eupatilin increases cilia incidence and length in CEP290 LCA10 patient fibroblasts. (A) Representative images of control (CEP290 WT) and CEP290 LCA10 fibroblasts treated with eupatilin (20 µM) or vehicle, stained for pericentrin (PCN, green, basal body) and ARL13b (magenta, axoneme). Scale bar: 20 µm. Inset shows cilia at higher magnification. Scale bar 5 µm. (B,C) Cilia incidence and length were scored using the elaboration of an ARL13B positive axoneme from a PCN positive basal body. Number of cilia counted in B: WT vehicle (-) n = 89, Eupatilin (+) n = 147; CEP290 LCA10 vehicle (-) n = 99, Eupatilin (+) n = 87 (B). Number of cilia counted in C: WT vehicle (-) n = 271, Eupatilin (+) n = 231; CEP290 LCA10 vehicle (-) n = 214, Eupatilin (+) n = 345. Mean ± standard deviation (SD), ** p < 0.01, *** p < 0.005, **** p < 0.0001 one-way ANOVA test.