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. 2023 Jun 7;12(12):1575. doi: 10.3390/cells12121575

Figure 3.

Figure 3

Eupatilin treatment restores ciliation and cilia length in iPSC-derived CEP290 KO and CEP290 LCA10 retinal organoids. (A) Bright field images of CEP290 retinal organoids at differentiation day 70. Scale bar: 500 µm. (B) RCVRN (green) and CRX (magenta) staining of day 120 retinal organoids. Scale bar: 40 µm. (C) Eupatilin treatment of retinal organoids from days 90 to 120. Cilia were stained with ARL13B (green) and PCN (magenta). Scale bar: 40 µm. Insets show a higher magnification of the photoreceptor cilia. Scale bar: 1 µm. (D) Quantification of photoreceptor ciliation and photoreceptor cilium length (E) in CEP290 retinal organoids at day 120 after eupatilin treatments. CEP290 WT DMSO n = 4 ROs (141 cilia), CEP290 WT EUP n = 4 ROs (135 cilia), CEP290 KO Vehicle n = 3 (148 cilia), CEP290 KO EUP n = 2 (88 cilia), CEP290 LCA10 Vehicle n = 4 (114 cilia), CEP290 LCA10 EUP n = 4 (176 cilia). * p < 0.05 ** p < 0.01, *** p < 0.001, ordinary one-way ANOVA with Tukey’s post hoc test.