Skip to main content
. 2023 Jun 7;12(12):1575. doi: 10.3390/cells12121575

Figure 4.

Figure 4

Opsin accumulates in the outer nuclear layer of CEP290 LCA10 retinal organoids and is rescued by eupatilin. (A) Representative images of CEP290 WT (control) and CEP290 LCA10 retinal organoids at D180 treated with eupatilin (20 µM) or vehicle for 30 days (from D150), stained for rhodopsin (magenta) and L/M opsin (green). Photoreceptor nuclei were stained with DAPI (blue). Scale bar: 10 µm. (B) Quantification of RHO and L/M opsin (C) immunofluorescence intensity in the photoreceptor outer nuclear layer (ONL). Each data point represents the mean of three measurements from one organoid, bar represents mean ± SD. * p < 0.05 ** p < 0.01, *** p < 0.001.