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. 2023 Jun 8;12(12):1591. doi: 10.3390/cells12121591

Figure 7.

Figure 7

Efficacy of single and double transfection of mRNAs. (A) Titration of the mRNA encoding for nano-Lamin-mScarlet after different incubation time points. MDA-MB231 cells were transfected with different amounts of mRNAs as indicated. Cells were fixed after 6, 12, 24, 48 and 72 h of incubation. Images were captured with fluorescence microscopy, and quantification was performed with ImageJ. Scale bar, 10 µm. (B) Double detection of lamin A and either eGFP-NLS or con1. MDA-MB231 cells were transfected with 50 ng of mRNA coding for nano-Lamin-mScarlet-E3 together with either 100 ng of mRNA coding for eGFP-NLS or 50 ng of con1-NLS-eGFP. After 24 h, the cells were fixed and analyzed with direct fluorescence microscopy. Scale bar, 10 µm. (C) Quantification with flow cytometry of single transfection of 100 ng of mRNA encoding for either nano-Lamin-mScarlet or con1-eGFP or together as indicated. The percentages of transfected positive cells are noted in each FACS plot.