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. 2023 Jun 8;24(12):9909. doi: 10.3390/ijms24129909

Figure 5.

Figure 5

Low CD56/MS4A4A EF ratios are associated with longer EFS. (a) Representative automated double CD56 (red) and MS4A4A (brown) bright-field IHC image. Scale bar 300 μm. (b–f) Box plots depicting the percentage of CD56/MS4A4A EF ratios, each dot represents the median value of 10 ROIs for every patient. Only values statistically significant are reported: * p < 0.05 (g) High CD56/MS4A4A EF ratios (≥18/HPF; n = 10) showed a worse EFS than those with lower values (n = 25). (h) Representative sequential IHC pseudo-color images show three distinct intratumoral macrophage populations: MS4A4A (blue), CD163 (red) and double-positive MS4A4A+CD163+ cells (magenta). Scale bar 200 μm. High magnification inserts on the right display single-marker expression and the relative color merge. PD, progressive disease/recurrent; FLIPI, Follicular Lymphoma international prognostic index; H/I, High/Intermediate; L, Low; POD24, progression of disease within 24 months; EFS, event-free survival.