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. 2023 Jun 20;14(6):1295. doi: 10.3390/genes14061295

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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VPA increases the cytotoxic effect of the DTIC alkylating agent and BMN-673 PARP1 inhibitor. DMBC11 cells were pretreated with VPA (1 mmol/L) for 168 h and then treated or not with BMN-673 (10 μM) and DTIC (2 mM). (A) Cell viability was evaluated with a trypan blue assay after 72 h of treatment. (B) Cell proliferation was evaluated with a clonogenic assay after 72 h of treatment. (C) Representative histograms of the apoptosis/necrosis analysis of melanoma cells after 24 h of treatment with DTIC and BMN-673 (and pretreatment with VPA). (D) The percentage of DMBC11 melanoma cells in early and late apoptosis. At least three independent experiments were performed, and the results are shown as the mean ± standard deviation (SD). * p ≤ 0.05, *** p ≤ 0.001, **** p-value ≤ 0.0001 compared with the control group.

VPA increases the cytotoxic effect of the DTIC alkylating agent and BMN-673 PARP1 inhibitor. DMBC11 cells were pretreated with VPA (1 mmol/L) for 168 h and then treated or not with BMN-673 (10 μM) and DTIC (2 mM). (A) Cell viability was evaluated with a trypan blue assay after 72 h of treatment. (B) Cell proliferation was evaluated with a clonogenic assay after 72 h of treatment. (C) Representative histograms of the apoptosis/necrosis analysis of melanoma cells after 24 h of treatment with DTIC and BMN-673 (and pretreatment with VPA). (D) The percentage of DMBC11 melanoma cells in early and late apoptosis. At least three independent experiments were performed, and the results are shown as the mean ± standard deviation (SD). * p ≤ 0.05, *** p ≤ 0.001, **** p-value ≤ 0.0001 compared with the control group.