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. 2023 Jun 20;14(6):1295. doi: 10.3390/genes14061295

Figure 2.

Figure 2

Figure 2

BMN673 and/or DTIC, VPA induced DSBs in melanoma cell lines (DMBC11). Cells were treated with 80 nM BMN-673 and/or 2 mM DTIC and VPA for 48 h (comet assay) and 120 hrs (γ-H2AX). (A) The mean percentage ± SD of DNA in the tails of comets in neutral conditions acquired from the 50 cells/group from 3 experiments. The characteristic tails of comets with damaged DNA are shown under a fluorescence microscope after electrophoresis and 4′,6-diamidino-2-phenylindole (DAPI) gel staining. (B) The mean values ± SD of γ-H2AX were calculated from 3 experiments performed in triplicate. The characteristic tails of comets with damaged DNA are shown under a fluorescence microscope after electrophoresis and 4′,6-diamidino-2-phenylindole (DAPI) gel staining. * p-value ≤ 0.05; *** p-value ≤ 0.001; **** p-value ≤ 0.0001 in comparison with the control.