Skip to main content
. 2023 Jun 10;24(12):9982. doi: 10.3390/ijms24129982

Figure 2.

Figure 2

CSE β-lyase activity toward cystine in cells. (A) Wild-type (WT), indicated mutants, or empty vector (Empty) were transfected into COS-7 cells and subjected to Western blotting. Anti-CSE and anti-GAPDH antibodies were used. (B) Live-cell fluorescence imaging of Cys-SSH produced in wild-type CSE or indicated mutants transfected COS-7 cells with SSP4. (C) Relative levels of Cys-SSH were estimated by quantification of the intensity of SSP4 in each sample in panel B. n = 50 were estimated by quantification of the intensity of SSP4 in each sample. Results are the mean ± SE. **** p < 0.0001 versus control sample as indicated. n.s.: not significant.