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Effect of C136V/C171V mutant on γ-lyase activities toward cystathionine to generate cysteine. Purified wild-type (WT) or C136V/C171V CSE was incubated with 50 µM PLP, 1 mM DTNB, and 1 mM cystathionine at 30 °C for 10 min, followed by measurement of absorbance at 412 nm, as described in the Materials and Methods section. Results are the mean ± SE of four independent experiments. n.s.: not significant.
