Table 2.

Primers used in PCR to detect Encephalitozoon spp., Neospora caninum and Toxoplasma gondii in bats collected in the Czech Republic and the Slovak Republic.

Primer Name	Oligonucleotide Sequences
Encephalitozoon cuniculi (ITS region)
MSP 1	5′ TGA ATG (G,T)GT CCC TGT 3′
MSP 2A	5′ TCA CTC GCC GCT ACT 3′
MSP 3	5′ GGA ATT CAC ACC GCC CGT C(A,G)(C,T) TAT 3′
MSP 4A	5′ CCA AGC TTA TGC TTA AGT (C,T)(A,C)A A(A,G)G GG 3′
Neospora caninum (Nc-5 region)
Np21+	5′ CCCAGTGCGTCCAATCCTGTAAC 3′
Np6+	5′ CTCGCCAGTCAACCTACGTCTTCT 3′
Toxoplasma gondii (529-bp repeated element)
TOX4	5′ CTGCAGGGAG GAAGACGAAA GTTG 3′
TOX5	5′ CGCTGCAGACACAGTGCATCTGGATT 3′