Table 3.

Microsatellite markers and primers used for Toxoplasma gondii genotyping by multiplex PCR assay.

Marker	Primer Sequence
TUB2	5′ 6-FAM-GTCCGGGTGTTCCTACAAAA 3′
	5′ TTGGCCAAAGACGAAGTTGT 3′
W35	5′ HEX-GGTTCACTGGATCTTCTCCAA 3′
	5′ AATGAACGTCGCTTGTTTCC 3′
TgM-A	5′ HEX-GGCGTCGACATGAGTTTCTC 3′
	5′ TGGGCATGTAAATGTAGAGATG 3′
B18	5′ 6-FAM-TGGTCTTCACCCTTTCATCC 3′
	5′ AGGGATAAGTTTCTTCACAACGA 3′
B17	5′ HEX-AACAGACACCCGATGCCTAC 3′
	5′ GGCAACAGGAGGTAGAGGAG 3′
M33	5′ 6-FAM-TACGCTTCGCATTGTACCAG 3′
	5′ TCTTTTCTCCCCTTCGCTCT 3′
IV.1	5′ HEX-GAAGTTCGGCCTGTTCCTC 3′
	5′ TCTGCCTGGAAAAGGAAAGA 3′
XI.1	5′ 6-FAM-GCGTGTGACGAGTTCTGAAA 3′
	5′ AAGTCCCCTGAAAAGCCAAT 3′
M48	5′ 6-FAM-AACATGTCGCGTAAGATTCG 3′
	5′ CTCTTCACTGAGCGCCTTTC 3′
M102	5′ NED-CAGTCCAGGCATACCTCACC 3′
	5′ CAATCCCAAAATCCCAAACC 3′
N60	5′ NED-GAATCGTCGAGGTGCTATCC 3′
	5′ AACGGTTGACCTGTGGCGAGT 3′
N82	5′ HEX-TGCGTGCTTGTCAGAGTTC 3′
	5′ GCGTCCTTGACATGCACAT 3′
AA	5′ NED-GATGTCCGGTCAATTTTGCT 3′
	5′ GACGGGAAGGACAGAAACAC 3′
N61	5′ 6-FAM-ATCGGCGGTGGTTGTAGAT 3′
	5′ CCTGATGTTGATGTAAGGATGC 3′
N83	5′ 6-FAM-ATGGGTGAACAGCGTAGACA 3′
	5′ GCAGGACGAAGAGGATGAGA 3′