Figure 1.
Chemical and effect-directed profiles of Gastrodia elata samples: G1–G5 (10 or 15 µg/band each) and chemical marker compounds gastrodin, vanillin and vanillyl alcohol (1 or 2 µg/band each) on HPTLC plates silica gel 60 developed with ethyl acetate–methanol–bidistilled water–formic acid (7.3/1.25/1.0/0.45, V/V/V/V) or HPTLC plates silica 60 RP-18 W developed with water–acetonitrile–methanol–formic acid (4.5/1/1/0.2, V/V/V/V), detected (a) at FLD 366 nm via the Fast Blue salt B reagent (not shown) followed by Natural Product reagent A and PEG on NP, or just at FLD 366 nm on RP, and at white light illumination after the (b) Fast Blue salt B reagent followed by 2-naphthol sulfuric acid reagent, (c) DPPH• assay, and (d) ABTS assay.
