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. 2023 Jun 11;24(12):10003. doi: 10.3390/ijms241210003

Figure 5.

Figure 5

Effects of Cr(VI) exposure on oxidative DNA damage (8-OHdG) in MII oocytes. Prepubertal rats were exposed to 1 or 5 ppm potassium dichromate through drinking water from PND 22 to 29 and superovulated. 8-OHdG, the biomarker of oxidative DNA damage, was determined by immunofluorescence in the MII oocytes, and images were captured by confocal microscopy. All images were captured with a 40×/1.4 NA Plan-Apochromat lens. The width of each field is 50 µm (A,C,D,F,G,I) or 15 µm (B,E,H; top panel) or 10 µm (B,E,H; bottom panel). Cr(VI) increased 8-OHdG expression. Representative images of the control (A–C), 1 ppm Cr(VI) (D–F), and 5 ppm Cr(VI) (G–I) groups are shown. The histogram (J) represents the intensity of staining (expressed as Integrated Optical Density (IOD)). Each value is mean ± SEM of ~24 oocytes from six rats (p < 0.05). a: control vs. Cr(VI) 1 ppm or 5 ppm. The red color represents chromosomes, and the green color represents 8-OHdG. Broken lines show 8-OHdG excision and migration towards the periphery of the oocytes (extracellular release).