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. 2023 Jun 8;24(12):9896. doi: 10.3390/ijms24129896

Figure 4.

Figure 4

Treatment with irisin prevents trabecular bone loss in the femurs of Ovx mice. (a) Representative micro-CT-generated section images and calculated cortical and trabecular parameters of femurs harvested from Sham-veh mice, Ovx-veh mice and Ovx-irisin mice. (b–f) Trabecular bone parameters included bone mineral density (BMD) in Sham-veh (n = 4), Ovx-veh (n = 5) and Ovx-irisin (n = 4) mice; bone volume/total volume (BV/TV) in Sham-veh (n = 12), Ovx-veh (n = 10) and Ovx-irisin (n = 10) mice; trabecular number (Tb.N) in Sham-veh (n = 12), Ovx-veh (n = 10) and Ovx-irisin (n = 10) mice; trabecular thickness (Tb.Th) in Sham-veh (n = 13), Ovx-veh (n = 10) and Ovx-irisin (n = 11) mice; and trabecular separation (Tb Sp) in Sham-veh (n = 11), Ovx-veh (n = 11) and Ovx-irisin (n = 10) mice. (g–i) Cortical bone parameters included tissue mineral density (TMD) in Sham-veh (n = 13), Ovx-veh (n = 11) and Ovx-irisin (n = 11) mice; cortical bone area (Ct. B.Ar) in Sham-veh (n = 12), Ovx-veh (n = 10) and Ovx-irisin (n = 11) mice; and cortical thickness (Ct.Th) in Sham-veh (n = 12), Ovx-veh (n = 11) and Ovx-irisin (n = 11) mice. (j,k) The parameters of the biomechanical properties included maximum load in Sham-veh (n = 10), Ovx-veh (n = 9) and Ovx-irisin (n = 9) mice and stiffness in Sham-veh (n = 6), Ovx-veh (n = 5) and Ovx-irisin (n = 7) mice. A one-way ANOVA with Tukey’s multiple comparisons tests was performed in (d–j). Kruskal–Wallis with Dunn’s multiple comparison tests was performed in (b,c,k). Data are presented as box-and-whisker plots with median and interquartile ranges, from max to min, with all data points shown. * p < 0.05, ** p < 0.01 vs. Sham-veh.