Figure 6.

Treatment with irisin decreases osteoclast number in trabecular bone of Ovx mice. (a) Representative images of tartrate-resistant acid phosphatase-stained (Trap positive) osteoclasts (black arrowhead) in vertebral sections. (magnification: 40×). (b) Analysis of osteoclast number per bone perimeter (OCs N./BP) in Sham-veh (n = 5), Ovx-veh (n = 6) and Ovx-irisin (n = 5) mice. (c) Concentration of serum bone resorption marker CTX determined using an ELISA assay in Sham-veh (n = 4), Ovx-veh (n = 4) and Ovx-irisin (n = 4) mice. A one-way ANOVA with Tukey’s multiple comparisons tests was performed in (c). Kruskal–Wallis with Dunn’s multiple comparison tests was performed in (b). Data are presented as box-and-whisker plots with median and interquartile ranges, from max to min, with all data points shown. * p < 0.05 vs. Sham-veh, # p < 0.05 vs. Ovx-veh.