Figure 3.

qPCR of L-PRLR, S-PRLR, and MAPK12 and the secretion of steroid hormones (E₂ and P₄). (A): Relative expression of L-PRLR and S-PRLR; (B): The secretion of steroid hormones (E₂ and P₄); (C): qPCR validation of L-PRLR after knockdown and overexpression; (D): qPCR validation of S-PRLR after knockdown and overexpression; (E): The relative expression of MAPK12 after knockdown and overexpression of L-PRLR and S-PRLR, respectively; (F): qPCR validation of MAPK12 after interference and overexpression. “*” and “**” indicate 0.01 < p < 0.05 and p < 0.01, respectively. L-PRLR: Long Prolactin Receptor; S-PRLR: Short Prolactin Receptor; C: Control group (0 ng/mL PRL); P: GCs treated with 500 ng/mL PRL (GCs with HPCs); P-458: GCs with HPCs were transfected by empty plasmid (negative control group for knockdown); P-sg-L: GCs with HPCs were transfected by recombinant plasmid of knockdown L-PRLR; P-sg-SL: GCs with HPCs were transfected by recombinant plasmid of both knockdown L-PRLR and S-PRLR; P-10: GCs with HPCs were infected by empty vectors of overexpression (negative control group for overexpression); P-10-L: GCs with HPCs were infected by lentiviruses carrying overexpressed sequences of L-PRLR; P-10-S: GCs with HPC were infected by lentiviruses carrying overexpressed sequences of S-PRLR; P-G: GCs with HPCs were infected by empty vectors of interference (negative control group for interference of MAPK12); P-SH1: GCs with HPCs were infected by lentiviruses carrying interfered sequence 1 of MAPK12; P-SH2: GCs with HPCs were infected by lentiviruses carrying interfered sequence 2 of MAPK12; P-SH3: GCs with HPCs were infected by lentiviruses carrying interfered sequence 3 of MAPK12; P-10-M: GCs with HPCs were infected by lentiviruses carrying overexpressed sequences of MAPK12.