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. 2023 Jun 17;24(12):10288. doi: 10.3390/ijms241210288

Figure 2.

Figure 2

Amino acid sequence and structure analysis of SbWRKY65. (A) Sequence alignment of SbWRKY65 and homologous proteins from other species, including Zea mays L. (ZmWRKY69, ONM38198.1), Setaria italica (L.) Beauv. (SiWRKY65, XP_004969837.1), Panicum virgatum L. (PvWRKY65, XP_039813594.1), Triticum aestivam L. (TaWRKY65, XP_044344816.1), Hordeum vulgare L. (HvWRKY65, XP_044972707.1), Oryza sativa L. (OsWRKY65, XP_015624912.1), Arabidopsis thaliana (L.) Heynh. (AtWRKY65, AT1G29590), and Nicotiana tabacum L. (NtWRKY65, XP_016511600.1). Horizontal lines indicate the WRKY motif and zinc finger motif. The asterisks indicate conserved Cys2His2 amino acids in zinc finger motif. (B) Phylogenic analysis of SbWRKY65 and the aforementioned homologous proteins. The phylogenetic tree was constructed according to the neighbor-joining method using MEGA 7. (C) Ribbon display model of WRKY domain (WRKY motif and zinc finger motif) of SbWRKY65. Purple ribbon on the left: WRKY motif with the residues (yellow) forming a H-bond (black dashed line). Brown sticks at the top: Cys or His residues with S or N atoms (brown balls) bound to a Zn2+ ion.