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. 2023 Jun 17;24(12):10288. doi: 10.3390/ijms241210288

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of subcellular localization and transcriptional activity of SbWRKY22 and SbWRKY65. (A) Subcellular localization of SbWRKY22 and SbWRKY65. Transient expression of GFP-SbWRKY22 and GFP-SbWRKY65 fusion proteins or GFP control in Arabidopsis protoplasts. DAPI, nuclear signal; GFP, GFP fluorescence; Bright, bright field. The scale bar is 20 μm. (B) Transcriptional activity of SbWRKY22 and SbWRKY65 in yeast. Y2HGold yeast strains carrying fused protein of GAL4 DNA-binding domain and SbWRKY22 (BD-SbWRKY22), SbWRKY65 (BD-SbWRKY65), or BD alone (vector) were cultured in SD/-Trp or SD/-Trp/-His medium.