Figure 6.

Overexpression of SbWRKY65 in transgenic Arabidopsis shows improved tolerance to Al stress. (A) Reverse transcription polymerase chain reaction characterization of SbWRKY65 expression in the overexpression lines. (B) The Al-sensitive phenotype of WT (Col-0) and the overexpression lines. Five-day-old seedlings were precultured on a solid MS medium at pH 5.8, then transferred to a solid medium containing 1 mM CaCl₂ and 1% (w/v) sucrose at pH 4.5, with or without 50 μM AlCl₃ for 5 d. Scale bar, 1 cm. (C) Relative root elongation (root elongation with Al treatment/root elongation without Al treatment) of WT and the overexpression lines. Data are shown as the means with SD (n ≥ 20). Columns with different letters indicate significant differences between plants according to Tukey’s test (p < 0.05). Experiments were repeated three times.