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. 2023 Jun 17;24(12):10288. doi: 10.3390/ijms241210288

Figure 7.

Figure 7

Transcriptional characteristic analysis of SbWRKY22. (A) Schematic diagram of the reporter and effector used in the dual-luciferase reporter system. pSbMATE, SbMATE promoter −2000 bp to −1 bp); pSbGlu1, SbGlu1 promoter (−2000 bp to −1 bp); pSbSTAR1, SbSTAR1 promoter (−1494 bp to −1 bp); pSbSTAR2a, SbSTAR2a promoter (−1678 bp to −1 bp); pSbSTAR2b, SbSTAR2b promoter (−1963 bp to −1 bp); LUC, firefly luciferase reporter; REN, Renilla luciferase reporter as an internal control; 35S, CaMV 35S promoter; Myc, protein tag. (B–F) Transcriptional regulation of SbMATE (B), SbGlu1 (C), SbSTAR1 (D), SbSTAR2a (E), and SbSTAR2b (F) by SbWRKY22 in the dual-luciferase reporter system. Luciferase activity of the reporter (LUC) driven by the promoters (pro) was normalized to the internal control reporter (REN). (G) Transcriptional regulation of SbMATE, SbGlu1, SbSTAR1, SbSTAR2a, and SbSTAR2b by SbWRKY22 in the yeast one-hybrid system. Scale bar, 0.5 cm. Data represent the means ± SD from three independent biological replicates. Asterisk (*) represents significant differences from the vector-only control according to Dunnett’s t test (p < 0.05). Asterisks (**) represent significant differences from the vector-only control according to Dunnett’s t test (p < 0.01).