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. 2023 Jun 17;24(12):10288. doi: 10.3390/ijms241210288

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Overexpression lines of SbWRKY22/SbWRKY65 show enhanced AtBG2/AtWRKY54 expression and reduced callose accumulation. (A,C) Quantitative real-time PCR analysis of the expression of AtBG2/AtWRKY54 in WT (Col-0) and two independent SbWRKY22/SbWRKY65 overexpression lines. Seven-day-old seedlings were pre-cultured on a solid MS medium vertically and then transferred to a liquid medium containing 0.5 mM CaCl₂ at pH 5.0 without AlCl₃ or with 15 μM AlCl₃ for 6 h. (B,D) Callose content in the roots of WT (Col-0) and two independent SbWRKY22/SbWRKY65 overexpression lines without or with 15 μM AlCl₃ for 6 h. Data represent the means ± SD for three independent biological replicates. Columns with different letters are significantly different according to Tukey’s test (p < 0.05).