Skip to main content
. 2023 Jun 15;24(12):10186. doi: 10.3390/ijms241210186

Figure 1.

Figure 1

Microscope system. (A) Optical scheme of the light-sheet microscope system. For illumination, the Bessel beam–forming unit was introduced. A Ti—sapphire laser oscillator, Insight Deepsee (700–1300 nm wavelength)—was used as the laser source. The illumination and the detection pathways were set perpendicular to each other. A Galvano mirror scanner was introduced to generate the light-sheet, and the created optical slice was imaged by a sCMOS camera. The sample mounting holder was controlled by the motorized xyzθ stages. For imaging, the image-splitting optics system W-View Gemini-2C (A12801-10, Hamamatsu Photonics, Hamamatsu, Japan) was used. The extended DOF device was inserted in the second pupil plane of the optical system. Abbreviations: HWP, half wavelength plate; GLP, glan-laser polarizers; M, mirror; BE, beam expander; D, doublet; L, lens; GS, Galvano mirror scanner; TfΘL, telecentric fΘ lens; DO, detection objective lens; TL, tube lens; F, emission filter; SSD, stair-step device. (B) Specification of the extended DOF device. The image (bottom) was acquired at the pupil plane with a Beltran lens.