Figure 4.

Comparison of transcriptomic data from glomerular monocultures and co-cultures between 2D and 3D culture conditions displays differences regarding cell type-specific marker, extracellular matrix and signaling components: (a) principal component analysis of glomerular cell monoculture and co-culture in 2D and 3D. Each condition was analyzed as a biological triplicate. Glomerular endothelial cell (GEC), mesangial cells (MC), co-culture of differentiated conditionally immortalized human podocytes, mesangial cells and glomerular endothelial cells (PEM); and (b–d) heatmaps representing gene expression of extracellular matrix components, cell type-specific markers, adhesion and signaling molecules of glomerular endothelial cell (GEC) monoculture (b), mesangial cell (MC) monoculture (c), and glomerular co-culture of podocytes, mesangial cells and glomerular endothelial cells (PEM) (d) in 2D and 3D. Each condition was analyzed as a biological triplicate.